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逐渐递减未作组份分离的人外周血单个核细胞的用量,发现细胞密度低于每管125×10~3后即无PHA诱发的体外增殖反应,然而此时增加没有增殖能力的外周血粘附细胞的含量可使上述反应得到恢复。由于尼龙毛纯化后的T细胞可再现上述因密度递减而出现的反应格局并同样显示了粘附细胞的重建现象,表明人体T细胞对PHA的增殖反应必须依赖具有粘附能力的大单核细胞。此外发现人混合血清也可发挥与粘附细胞相似的协助作用,且效果更佳。然而血清的这一功能及其最大协助反应值的出现必须以原有的或进一步加入的少量粘附细胞存在为前提。与此同时,重建过程中没有血清的参与仅加粘附细胞也远不能出现最强的增殖反应。说明PHA诱发的人体外周血淋巴细胞转化需要三种成份,即T细胞、大单核细胞和血清三者之间的相互作用。
The amount of peripheral blood mononuclear cells that had not been fractionated was gradually decreased and the cell density was found to be lower than that of no PHA-induced in vitro proliferative response after each 125 × 10 -3 tube. However, peripheral blood adhesion without proliferation was increased With the cell content can make the above reaction was restored. Since the purified T nylon cells can reproduce the above reaction pattern due to the decreasing density and also show the remodeling phenomenon of adherent cells, it indicates that the proliferation response of human T cells to PHA must rely on the adhesion capacity of large mononuclear cells . In addition, we found that human mixed serum can also play a similar role as adherent cells, and the effect is better. However, this function of serum and its maximal assist response value must be premised on the presence of a small amount of adherent cells, either existing or further added. At the same time, the strongest proliferative response is far from being able to occur with no serum involvement in the reconstitution process alone with adherent cells. It shows that PHA-induced human peripheral blood lymphocyte transformation requires three components: the interaction between T cells, macrophages and serum.