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本文报道应用人结肠癌建株LoVo细胞接种裸鼠,并鼠间移植传代,建立人结肠癌(HCC)裸鼠移植瘤模型。接种和传代的移植瘤具有相对稳定性,其成活率达100％。通过对移植瘤大体形态观察和病理组织学、兔疫组化、透射电镜检查,以及~(131)I标记抗CEA单抗的体内放免定位显像和免疫毒素导向抗肿瘤一系列实验研究表明这一模型无论从形态结构、病理特征、生物学标志和免疫学功能都与原发性肿瘤极为相似,因此对于肿瘤细胞胚胎基因再表达机制和肿瘤抗原的分析,以及肿瘤的实验诊断和导向治疗研究应用具有重要意义。
This paper reports the application of human colon cancer LoVo cells inoculated into nude mice and transplantation of mice to establish a human colon cancer (HCC) xenograft tumor model in nude mice. The transplanted tumors that were inoculated and passaged were relatively stable and had a survival rate of 100%. Through the observation of gross morphology of transplanted tumors, histopathology, radioimmunohistochemistry and transmission electron microscopy, as well as a series of experimental studies of ~(131)I-labeled anti-CEA monoclonal antibody in vivo RIA and immunotoxin-directed anti-tumor experiments. A model is very similar to primary tumors in terms of morphological structure, pathological features, biological markers, and immunological functions. Therefore, the analysis of tumor cell embryonic gene re-expression and tumor antigens, as well as experimental diagnosis and targeted therapy of tumors The application is of great significance.