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Objective To investigate the influence of hepatitis B virus X gene(HBx)on apoptosis of hepatic cells mediated by Fas in Hep G2 cells.Methods HBx eukaryotic vector pc DNA3.1(+)-X was transfected into HEPG2cells with lipofectamine,and the null vector pc DNA3.1(+)and untransfected HEPG2 were used as normal controls.The cells were collected 72 h after transfection,and the expression of HBx mRNA and protein was deter-
Objective To investigate the influence of hepatitis B virus X gene (HBx) on apoptosis of hepatic cells mediated by Fas in Hep G2 cells. Methods HBx eukaryotic vector pc DNA3.1 (+) - X was transfected into HEPG2 cells with lipofectamine, and the null vector pcDNA3.1 (+) and untransfected HEPG2 were used as normal controls. The cells were collected for 72 h after transfection, and the expression of HBx mRNA and protein was deter-