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目的观察不同浓度的葡萄糖和胰岛素对结直肠癌细胞株Colon26肿瘤细胞分泌白介素6(IL-6)的影响。方法体外常规培养Colon26肿瘤细胞。根据所用培养液外加葡萄糖和胰岛素终浓度的不同,将实验所用细胞分为4组(12亚组),培养48 h,收集细胞培养上清,ELISA法检测IJ-6浓度。结果葡萄糖浓度为25mmol/L的亚组和其加入不同浓度的胰岛素的亚组细胞培养上清中IL-6的浓度明显高于对照组(P<0.01);其他亚组与对照组相比均无显著差异(P>0.05)。结论 25mm0l/L的葡萄糖可显著增加Colon26肿瘤细胞分泌IL-6;不同浓度的胰岛素对Colon26肿瘤细胞分泌IL-6无明显影响。
Objective To observe the effect of different concentrations of glucose and insulin on the secretion of Interleukin 6 (IL-6) in colorectal cancer cell line Colon26. Methods Colon26 tumor cells were cultured in vitro routinely. The cultured cells were divided into 4 groups (12 subgroups) according to different concentrations of final glucose and insulin. The medium was cultured for 48 h, and the supernatant of cell culture was collected. The concentration of IJ-6 was detected by ELISA. Results The concentrations of IL-6 in the supernatant of 25 mmol / L glucose group and its subgroups with different concentrations of insulin were significantly higher than those in the control group (P <0.01) No significant difference (P> 0.05). Conclusion 25mm0l / L glucose can significantly increase Colon26 tumor cells secrete IL-6; Different concentrations of insulin on Colon26 tumor cells secrete IL-6 had no significant effect.