Background: High-intensity interval training (HIIT) induces similar or even superior adaptations compared to continuous endurance training. Indeed, just 6 HIIT sessions over 2 weeks significantly improves maximal oxygen uptake (VO2max), submaximal exercise fat oxidation, and endurance performance. Whether even faster adaptations can be achieved with HIIT is not known. Thus, we aimed to determine whether 2 ses-sions of HIIT per day, separated by 3 h, every other day for 5 days (double HIIT (HIIT-D), n=15) could increase VO2max, submaximal exercise fat oxidation, and endurance capacity as effectively as 6 sessions of HIIT over 2 weeks (single HIIT (HIIT-S), n=13). Methods: Each training session consisted of 10×60 s of cycling at 100% of VO2max interspersed with 75 s of low-intensity cycling at 60 watt (W). Pre-and post-training assessments included VO2max, time to exhaustion at～80% of VO2max, and 60-min cycling trials at～67% of VO2max. Results: Similar increases (p＜0.05) in VO2max (HIIT-D: 7.7% vs. HIIT-S: 6.0%, p＞0.05) and endurance capacity (HIIT-D: 80.1% vs. HIIT-S:79.2%, p ＞ 0.05) were observed. Submaximal exercise carbohydrate oxidation was reduced in the 2 groups after exercise training (HIIT-D:9.2%, p=0.014 vs. HIIT-S: 18.8%, p=0.012) while submaximal exercise fat oxidation was significantly increased in HIIT-D (15.5%, p=0.048) but not in HIIT-S (9.3%, p=0.290). Conclusion: Six HIIT sessions over 5 days was as effective in increasing VO2max and endurance capacity and was more effective in improving submaximal exercise fat oxidation than 6 HIIT sessions over 2 weeks.