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迄今,已知可产生腹泻原性毒素的细菌有:霍乱弧菌、产毒性大肠杆菌、难辨梭状芽胞杆菌、非 O1群霍乱弧菌、副溶血性弧菌、河弧菌、沙门氏菌、耶尔森氏菌、克雷白氏菌、产气单胞菌、产气梭状芽胞杆菌、葡萄球菌和空肠弯曲菌等。作者推荐用反向被动血凝试验法(RP-HA)检测产毒性大肠杆菌的不耐热肠毒素(LT)。其具体操作步骤是:挑取1白金耳的病人粪便接种在 CAYE 3培养基上,37℃培养过夜,在培养基上加多粘菌素B0.1ml(2万单位/ml),再经37℃培养3小时,加1ml 磷酸盐缓冲液,用手摇混合器搅拌,离心20分钟(每分钟3,000转),取其上清液进行检测。用一般培养法,17个检样中有16个检出产生 LT 的细菌,22个检样中有19个检出产生 LT+ST(耐热性肠毒素)的细
To date, the bacteria known to produce diarrhea-origin toxins are: Vibrio cholerae, Toxigenic Escherichia coli, Clostridium difficile, Vibrio cholera non-O1, Vibrio parahaemolyticus, Vibrio cholerae, Salmonella, Corynebacterium, Klebsiella, Aeromonas, Clostridium gasgenii, Staphylococcus and Campylobacter jejuni. The authors recommend the use of reverse passive hemagglutination test (RP-HA) to detect heat-labile enterotoxin (LT) of toxigenic E. coli. The specific steps are: pick a platinum ear patient inoculated in CAYE 3 medium, 37 ° C overnight culture, add to the medium polymyxin B0.1ml (20,000 units / ml), and then 37 C for 3 hours, add 1 ml of phosphate buffer, stir with a hand mixer, centrifuge for 20 minutes (3,000 revolutions per minute), and take the supernatant for testing. With the general culture method, 16 of the 17 samples were found to produce LT, and of the 22 samples, 19 were found to produce LT + ST (heat-tolerant enterotoxin)