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目的:观察被动吸烟加气管滴注脂多糖(LPS)对大鼠肺组织损伤及肺组织和血清中SOD活力的影响,探讨被动吸烟加气管滴注LPS在大鼠慢性气道炎性反应中的作用。方法:32只大鼠随机分为对照组和低、中、高浓度组。对照组和低、中、高浓度组分别设定烟雾浓度为0%、50%、70%、90%,低、中、高浓度组于第7天气管滴注LPS,对照组滴等量0.9%氯化钠溶液。观察记录被动吸烟前后大鼠一般状况、肺脏及气管病理变化及肺组织和血清中SOD活力改变情况。结果:吸烟组大鼠的肺组织损伤及炎症病理学总评分及各项目评分显示高于对照组(P<0.05);高浓度组评分高于低浓度组(P<0.05)。吸烟组大鼠气管病理损伤程度重于对照组。吸烟组大鼠肺组织及血清中SOD的组活力均降低(P<0.05),与吸烟浓度成相关性。结论:被动吸烟加气管滴注LPS导致大鼠组织损伤及SOD活力降低,其引起的病变反映类似慢性阻塞性肺疾病形态学改变。
OBJECTIVE: To observe the effects of passive smoking with LPS on lung tissue injury and SOD activity in lung tissue and serum in rats, and explore the relationship between passive smoking and LPS in chronic airway inflammatory reaction in rats effect. Methods: Thirty-two rats were randomly divided into control group and low, medium and high concentration groups. The control group and the low, medium and high concentration groups were set to 0%, 50%, 70%, 90% respectively. In the low, medium and high concentration groups, LPS was dropped on the seventh day, % Sodium chloride solution. Observe and record the general condition of rats before and after passive smoking, lung and tracheal pathological changes and lung tissue and serum SOD activity changes. Results: The lung injury and inflammatory pathology score and the score of each item in smoking group were significantly higher than those in control group (P <0.05). The score of high concentration group was higher than that of low concentration group (P <0.05). The degree of tracheal injury in smoking group was heavier than that in control group. The smoking group rats lung tissue and serum SOD activity were lower (P <0.05), and smoking concentration into a relevant. CONCLUSION: Passive smoking and intratracheal intratracheal instillation of LPS results in the reduction of tissue injury and SOD activity in rats, and the pathological changes induced by LPS can reflect the morphological changes similar to those of chronic obstructive pulmonary disease.