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选用Ⅲ型脊髓灰质炎病毒 ,3批活疫苗样品 (WHO/Ⅲ ,参考疫苗 ;93/ 36 3和 3J 2批猴体神经毒力实验不合格的疫苗 )和 1株标准强毒株 (Leon) ,脊髓内注入携带有人细胞脊髓灰质炎病毒受体基因的转基因小鼠 (PVRTg2 1)。临床观察和组织病理学检查表明 ,Leon病毒的毒力极强 ,2 .0LogTCID50 可使 10 0 %小鼠麻痹和死亡。WHO/Ⅲ参考疫苗毒力最弱 ,5 .5LogTCID50 才能使 81.7%小鼠麻痹和 5 1.7%小鼠死亡。另 2种疫苗样品的毒力都高于参考制品。各项观察指标随毒力的不同而有明显差别 ,与猴体神经毒力实验结果一致。证明PVRTg2 1小鼠可以用作评价脊髓灰质炎活疫苗毒力试验的动物模型 ,也可用于病毒学和流行病学研究
Three types of poliovirus (WHO / Ⅲ, reference vaccines; 93/36 3 and 3J batches of unqualified neurovirulence vaccine) and one standard virulent strain (Leon) were selected. , A transgenic mouse (PVRTg2 1) carrying the human cell poliovirus receptor gene was injected into the spinal cord. Clinical observations and histopathological examination showed that the virulence of Leon virus is very strong and 2.0 LogTCID50 can paralyze and kill 100% of mice. The WHO / III reference vaccine had the weakest virulence with 5.5 LogTCID50 to paralyze 81.7% of mice and 5 1.7% of mice died. The other two vaccine samples have higher virulence than the reference product. The observation indicators vary significantly with the virulence, consistent with the results of the monkey virulence test. It has been demonstrated that PVRTg2 1 mice can be used as an animal model to evaluate virulence tests on live poliomyelitis vaccine as well as in virological and epidemiological studies