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目的:检测Livin蛋白在妊娠滋养细胞疾病中的表达,探讨其在妊娠滋养细胞疾病发生、发展中的作用。方法:收集2006年6月～2008年6月间山东省聊城市人民医院妇产科门诊手术或住院病人127例的组织标本,其中正常早孕绒毛组织35例、葡萄胎组织42例、侵蚀性葡萄胎组织28例及绒癌组织22例。采用免疫组化链酶抗生物素-过氧化物酶(SP)法对各组织标本中Livin蛋白的表达进行检测。结果:Livin蛋白在正常早孕组、葡萄胎组、侵蚀性葡萄胎组及绒癌组中滋养细胞细胞核及细胞浆的表达强度依次增加,各组间的表达强度差异有统计学意义(P<0.05)。结论:Livin蛋白在滋养细胞的过度表达在滋养细胞恶性病变的发生、发展中发挥重要作用。
Objective: To detect the expression of Livin protein in gestational trophoblastic disease and to explore its role in the occurrence and development of gestational trophoblastic disease. Methods: Tissue samples of 127 cases of obstetrics and gynecology outpatient or inpatient in Liaocheng People’s Hospital of Shandong Province from June 2006 to June 2008 were collected. Among them, 35 cases of normal early pregnancy villi, 42 cases of hydatidiform mole, 28 cases of fetal tissue and 22 cases of choriocarcinoma. Immunohistochemical streptavidin-peroxidase (SP) method was used to detect the expression of Livin in various tissues. Results: The expression of Livin protein in nuclei and cytoplasm of gestational trophoblast increased in turn in normal pregnancy group, hydatidiform mole group, invasive hydatidiform mole group and choriocarcinoma group, and the expression intensity of Livin protein in each group had statistical significance (P <0.05 ). CONCLUSION: Overexpression of Livin in trophoblast cells plays an important role in the occurrence and development of trophoblastic malignant lesions.