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目的探讨肿瘤坏死因子超家族成员4(TNFSF4)基因rs505922位点单核苷酸多态性(SNP)与动脉粥样硬化性脑梗死发病的关系。方法选取2012年1月至2013年12月收治的185例山东省滕州市户籍的动脉粥样硬化性脑梗死患者为缺血性脑卒中组,另选取180名行常规检查的正常体检者为对照组。采用PCR-RFLP法测定两组TNFSF4基因上SNP rs505922的基因频率及多态性。结果 rs505922基因型及基因频率分析表明缺血性脑卒中组C等位基因频率高于对照组,差异有统计学意义(P<0.05)。经Logistic多因素分析可知rs505922 CC型是动脉粥样硬化性脑梗死发病风险的独立危险因子。结论 TNFSF4基因位点上SNP rs505922 CC型与滕州地区动脉粥样硬化性脑梗死关系密切。
Objective To investigate the association between rs505922 SNP and the incidence of atherosclerotic cerebral infarction in tumor necrosis factor superfamily member 4 (TNFSF4) gene. Methods A total of 185 patients with atherosclerotic cerebral infarction registered in Tengzhou, Shandong Province from January 2012 to December 2013 were selected as ischemic stroke group and 180 normal controls were selected as controls group. The frequency and polymorphism of SNP rs505922 in TNFSF4 gene were determined by PCR-RFLP. Results The rs505922 genotype and frequency of gene showed that the frequency of C allele in ischemic stroke group was higher than that in control group (P <0.05). Logistic multivariate analysis showed that rs505922 CC type was an independent risk factor for the risk of developing atherosclerotic cerebral infarction. Conclusion The SNP rs505922 CC genotype at TNFSF4 locus is closely related to atherosclerotic cerebral infarction in Tengzhou area.