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目的:探讨强迫游泳引起的镇痛效应与中脑导水管周围灰质(PAG)中脑啡肽能神经元的关系。方法:12只雄性前脑啡肽原-绿色荧光蛋白(PPE-GFP)转基因小鼠随机分为两组:对照组(Control)和应激组(Stress),采用热板测痛仪测定小鼠的热痛阈;应用免疫荧光双标染色技术检测两组小鼠PAG中Fos阳性神经元、Fos/GFP双标神经元的分布模式及数量变化。结果:应激组小鼠应激后10 min,热痛阈明显升高到峰值,30 min时逐渐下降,在60 min时降到应激前时的基础值。免疫荧光染色结果显示:应激组小鼠在应激后2 h,PAG内Fos阳性神经元和Fos/GFP双标神经元明显增多,主要集中于PAG腹外侧区。结论:强迫游泳应激可在短期内引起一定程度的镇痛效应,这可能与其激活了PAG腹外侧区的脑啡肽能神经元有关。
Objective: To investigate the relationship between the analgesic effect caused by forced swimming and the enkephalingic neurons in periaqueductal gray (PAG). Methods: Twelve male PPE-GFP transgenic mice were randomly divided into two groups: control group and stress group, and the mice . The immunofluorescence double-stained staining was used to detect the distribution pattern and quantity of Fos positive neurons and Fos / GFP double-labeled neurons in PAG mice. Results: At 10 minutes after stress, the heat pain threshold increased significantly to peak value at 30 min and gradually decreased at 30 min, and decreased to the pre-stress baseline value at 60 min. Immunofluorescence staining showed that the Fos-positive neurons and Fos / GFP double-labeled neurons were significantly increased in stress-induced mice 2 h after stress, and mainly concentrated in the ventral region of PAG. Conclusion: Forced swimming stress can induce a certain degree of analgesic effect in a short period of time, which may be related to the activation of enkephalinine neurons in the ventral part of PAG.