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1983～1986年间,我们开展了利用茎尖微芽嫁接脱除柑桔黄龙病病原的研究。结果从嫁接的85株苗中,获得3株茎尖嫁接苗。对茎尖嫁接苗和原病株(对照)进行生物学鉴定和电子显微镜检查,示明以茎尖嫁接苗的多芽枝段接种的6株椪柑实生苗,均无表现病状;在电镜下在其叶脉的筛管细胞内均无发现病原体。反之,用对照病株的多芽枝段接种的9株椪柑实生苗,经过5～9月,全部表现典型的黄龙病病状;在电镜下在其叶脉的筛管细胞内则观察到很多黄龙病的特征性的病原体。这说明应用茎尖嫁接法可成功地脱除母树携带的黄龙病病原,获得无病的柑桔植株。
From 1983 to 1986, we carried out the use of shoot tip micro-bud grafting citrus Huanglongbing pathogen study. Results Three shoot tips were obtained from 85 grafted plants. The grafted seedlings of the tip of the shoots and the original strain (control) were identified by biology and electron microscopy, which showed that the six seedlings of Citrus reticulata seedlings inoculated with shoot branches of shoot-tip grafted seedlings showed no symptoms. Under electron microscope No pathogen was found in the veins of the screened cells. On the contrary, the 9 citrus seedlings inoculated with the branches of the control strain showed typical symptoms of yellow dragon’s disease after 5 ~ 9 months. A great number of yellow dragon’s disease The characteristic pathogen. This shows that the application of shoot-tip grafting method can successfully remove the pathogen of yellow dragonfly carried by the mother tree and obtain the disease-free citrus plant.