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在盆栽试验中，使用差的缓冲沙土比较Ｓｕｂｃｌｏｖｅｒ，Ｍｅｄｉｃｓ，Ｓｅｒｒａｄｅｌａ，Ｂｉｓｅｒｒｕｌａ和Ｗｏｏｌｙｃｌｏｖｅｒ固氮的酸化潜力，植物生长７、９、１１周后，这些豆科牧草的酸化率，地上部分４４～１４５ｃｍｏｌ／ｋｇ种间变化非常大．Ｓｕｂｃｌｏｖｅｒ和Ｓｅｒｒａｄｅｌａ比Ｗｏｏｌｙｃｌｏｖｅｒ和Ｍｅｄｉｃｓ表现较大的酸化能力，而Ｂｉｓｅｒｕｌａ酸化能力最低，酸化率在地上部不仅与过量阳离子和钙浓度有关，而且与灰碱有关．因此，地上部分总钙，过量阳离子和灰碱含量为土壤产生总的Ｈ＋提供了好的指示．
In pot experiments, the acidification rates of these leguminous forages were compared with those of Subclover, Medics, Serradela, Biserrula and Woolyclover after 7, 9, and 11 weeks of plant growth by using poor buffer sandy soils. The acidification rates of these leguminous forages ranged from 44 to 145 cmol / kg Variety is very large. Subclover and Serradela exhibited greater acidification capacity than Woolyclover and Medics, while Biserula had the lowest acidification rate. The acidification rate was above-ground in relation to excess cation and calcium concentration and was related to gypsum. Therefore, above-ground total calcium, excess cations and ash content provide good indications of total H + production in the soil.