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采用GC/MS法对江苏沿海四种低值贝类软体中脂肪酸类成分进行定性分析,建立了HPLC法测定4种贝类软体中多烯不饱和脂肪酸二十碳五烯酸(简称EPA)和二十二碳六烯酸(简称DHA)的方法,在此基础上分析不同采收期4种贝类软体EPA和DHA的动态积累规律以评价这4种贝类软体的适宜采收期;同时以Waters Sun Fire C18柱(2.1 mm×100 mm,3.5μm)为色谱柱,乙腈(含0.1%磷酸)-水(含0.1%磷酸)(体积比74∶26)为流动相,流速0.6 m L/min等度洗脱,检测波长205 nm。该方法下,EPA-甲酯和DHA-甲酯分别在1.005 0~80.40μg/m L、1.106 2~88.50μg/m L范围内呈良好的线性关系,灵敏度高,重现性好。不同的采收期,4种贝类软体中EPA和DHA的含量变化明显,在6.7月份时达到最高,菲律宾蛤仔、文蛤、青蛤和四角蛤蜊软体中EPA和DHA的总量分别为:771.56~4 565.38μg/g、263.11~3 115.78μg/g、5 907.83~12 023.06μg/g和422.71~2 416.27μg/g。从产量和有效成分EPA和DHA的总量变化上综合考虑,建议菲律宾蛤仔、青蛤和四角蛤蜊最佳采收期在6月,而文蛤最佳采收期在7月。
The qualitative and quantitative analysis of fatty acids in four low-molluscan shellfish shells by using GC / MS method was established. The HPLC method for the determination of polyunsaturated fatty acid eicosapentaenoic acid (referred to as EPA) and Docosahexaenoic acid (referred to as DHA) method, on the basis of the analysis of different harvesting period of four kinds of molluscs EPA and DHA dynamic accumulation rules to evaluate the four kinds of shellfish suitable harvest time; at the same time Acetonitrile (containing 0.1% phosphoric acid) -water (containing 0.1% phosphoric acid) (74:26 by volume) as the mobile phase on a Waters Sun Fire C18 column (2.1 mm × 100 mm, 3.5 μm) / min isocratic elution, detection wavelength 205 nm. Under this method, EPA-methyl ester and DHA-methyl ester showed good linearity in the range of 1.005 0 ~ 80.40μg / m L and 1.106 2 ~ 88.50μg / m L respectively, with high sensitivity and reproducibility. The contents of EPA and DHA in four shellfish mollusks varied significantly at different harvest times and reached their peak at 6.6 months. The total amounts of EPA and DHA in the clams of Ruditapes philippinarum, Clams, Cyclops and Tetrahedron clams were 771.56 ~ 4 565.38 μg / g, 263.11 ~ 3 115.78 μg / g, 5 907.83 ~ 12 023.06 μg / g and 422.71 ~ 2 416.27 μg / g. Considering the changes of total output and active ingredients EPA and DHA, we suggest that the best harvest period of clams, blue clams and clams in June is in June, while the best harvest time of clams is in July.