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目的:鉴定零陵香水溶性多糖组分,为建立专属性的质量评价指标和中药药效组分新药研发奠定基础。方法:采用水提醇沉法制备零陵香粗多糖,Sevag法除蛋白、H2O2脱色、透析得到精制多糖,衍生化后用GC-MS分析。结果:零陵香水溶性多糖主要组分依次为鼠李糖醇-阿拉伯糖-核糖-葡萄糖-木糖,其相对含量比例为1.03∶9.17∶4.74∶18.95∶17.11。结论:GC-MS联用法可快速鉴定零陵香水溶性多糖组分。
OBJECTIVE: To identify the constituents of Lingling perfume soluble polysaccharides, and to lay the foundation for the establishment of a specific quality evaluation index and the research and development of new drugs for Chinese medicinal components. Methods: The crude polysaccharides of Lingolinggan were prepared by water-extraction and alcohol precipitation method. The proteins were purified by Sevag method and bleached by H2O2. The purified polysaccharides were obtained by dialysis, and were analyzed by GC-MS after derivatization. Results: The main components of Lingling soluble polysaccharides were rhamnitol - arabinose - ribose - glucose - xylose, the relative content ratio of 1.03: 9.17: 4.74: 18.95: 17.11. Conclusion: GC-MS method can be used to quickly identify components of Lingling perfume soluble polysaccharides.