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Chinese fir [Cunninghamia lanceolata(Lamb.)Hook] is a major timber tree species in China.In the present study,sequence-related amplified polymorphism(SRAP) markers were used to evaluate the polymorphisms,diversity,and relationships in a collection of Chinese fir elite genotypes(n=103) from the Lechang provenance,an endangered provenance.Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes;577(93.1 %) of these were polymorphic,yielding an average of 16.5 polymorphic bands per primer combination.Of the SRAP primer combinations(n=35),14 ones identified C70.0 % of the 120 genotypes.The Me12–Em19 combination had the strongest discriminatory ability with 93.3 % of the genotypes identified.An UPGMA dendrogram further showed that most of the Lechang provenance genotypes(87.4 %) grouped together(Cluster A).The Lechang genotypes had a considerable amount of genetic variation with genetic similarity from 0.40 to 0.81.The current work may facilitate the management of the endangered Lechang Chinese fir provenance.
Chinese fir [Cunninghamia lanceolata (Lamb.) Hook] is a major timber tree species in China. The present study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the polymorphisms, diversity, and relationships in a collection of Chinese Five elite genotypes (n = 103) from the Lechang provenance, an endangered provenance.Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes; 577 (93.1%) of these were polymorphic , an average of 16.5 polymorphic bands per primer combination. Of the SRAP primer combinations (n = 35), 14 ones identified C70.0% of the 120 genotypes.The Me12-Em19 combination had the strongest discriminatory ability with 93.3% of the genotypes identified. An UPGMA dendrogram further showed that most of the Lechang provenance genotypes (87.4%) grouped together (Cluster A). The Lechang genotypes had a considerable amount of genetic variation with genetic similarity fro m 0.40 to 0.81 The current work may facilitate the management of the endangered Lechang Chinese fir provenance.