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中华猕猴桃(Actinidia chinensis Planch.)蔓尖用标准方法表面消毒后,在仍受两种具活性的细菌污染的情况下,在MS培养基上培养。液体培养基内培养物鲜重的增量及产生的茅数均大于用0.4％或0.8％琼脂固化培养基的。用125亳升锥形瓶培养产生的芽数多于用50、250、或500毫升的。6-苄氨基嘌呤的浓度为2亳克/升时,鲜重的增量比用0或4亳克/升的大。自增殖培养物切下的小苗在0.05％吲哚丁酸溶液中浸渍后直接插入泥炭、蛭石和珍珠岩的混合土内诱导生根。在907棵小植株中,93％以上驯化成功。此法的培养效果比得上用分生组织进行无菌培养的效果。因此,经最初表面消毒后存活的两种污染菌并没有对猕猴桃的离体繁殖产生严重影响。
Actinidia chinensis Planch., Was surface-sterilized by standard methods and cultured on MS medium while still contaminated by two active bacteria. The increase of fresh weight and the number of produce of the culture in the liquid medium were all greater than those of the medium hardened with 0.4% or 0.8% agar. Cultivation with a 125 ml Erlenmeyer flask produces more sprouts than 50, 250, or 500 ml. When the concentration of 6-benzylaminopurine is 2 mg / L, the fresh weight increase is greater than that of 0 or 4 mg / L. Seedlings cut from proliferative cultures were immersed in 0.05% indole butyric acid solution and directly inserted into the mixed soil of peat, vermiculite and perlite to induce rooting. Of the 907 plantlets, more than 93% were domestically successful. The cultivation of this method is comparable to the effect of aseptic culture with meristem. Therefore, the two contaminated bacteria that survived the initial surface disinfection did not have a serious impact on the in vitro propagation of kiwifruit.