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以自育的S型细胞质雄性不育系MD32为供体,京科968的母本京724为受体,利用回交转育结合分子标记辅助背景选择,仅回交3代即获得京724遗传背景的S型细胞质雄性不育系。经花粉I2-KI染色鉴定,京724不育系花粉败育完全。
Using selfing-type S-type cytoplasmic male sterile line MD32 as donor and Jingke 968 as female parent Jing 724 as receptor, backcrossing and breeding combined with molecular marker assisted background selection, Background of S-type cytoplasmic male sterile lines. Pollen I2-KI staining identification, Beijing 724 male sterile pollen abortion completely.