以广寄主范围质粒ｐＵＣＰ１８和ｐＵＣＰ１９为载体，用电激转化法，将苏云金杆菌杀虫蛋白基因ｃｒｙｌＡ（ｃ）片段导入了荧光假单胞菌Ｐ３０３菌株。Ｓｏｕｔｈｅｒｎ印迹分析和Ｗｅｓｔｅｒｎ印迹分析分别证实ｃｒｙｌＡ（ｃ）基因的导入和晶体蛋白在Ｐ３０３中的表达。生物活性测定结果表明，新构建的荧光假单胞工程菌株不仅保持了野生型自然菌株对小麦全蚀病有良好的抑菌活性，而且表现出对小菜蛾、玉米螟有显著的毒杀作用。 The crylA (c) fragment of the insecticidal protein gene of Bacillus thuringiensis was introduced into Pseudomonas fluorescens P303 strain by using the host-wide plasmids pUCP18 and pUCP19 as vectors. Southern blot analysis and Western blot analysis confirmed the crylA (c) gene import and crystal protein expression in P303, respectively. The bioassay results showed that the newly constructed Pseudomonas fluorescens engineering strain not only maintained the wild type natural strain has good antibacterial activity on wheat eclipse, but also showed significant toxicity to diamondback moth and corn borer.