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嗜线虫致病杆菌(Xenorhabdus nematophila HB310)和发光杆菌(Photorhabdus luminescens HB140)是分别从小卷蛾斯氏线虫和异小杆线虫中分离到的2株昆虫病原线虫共生菌。为了明确这2种共生菌的抑菌活性,分别测定了其对苹果上6种主要病原真菌的抑菌活性。结果表明,这2种共生菌对6种苹果病原真菌均有不同程度的抑制作用。嗜线虫致病杆菌HB310菌株发酵液对苹果轮纹病病原菌抑制效果最好,抑制率达76.9%,对其他病原菌的抑制作用依次为苹果树腐烂病病原菌(74.1%)>苹果斑点落叶病病原菌(60.9%)>苹果灰霉病病原菌(46.7%)>苹果青霉病病原菌(44.5%)>苹果炭疽病病原菌(23.0%);发光杆菌HB140菌株发酵液对苹果灰霉病病原菌的抑制作用最好,抑制率达到86.7%,对其他病原菌的抑制作用依次为苹果树腐烂病病原菌(76.6%)>苹果轮纹病病原菌(58.8%)>苹果斑点落叶病病原菌(46.0%)>苹果青霉病病原菌(44.2%)>苹果炭疽病病原菌(39.9%)。2种共生菌发酵液对苹果轮纹病菌均有较强的抑制作用,其EC50分别为42.28和42.92 mL/L。
Xenorhabdus nematophila HB310 and Photorhabdus luminescens HB140 are two strains of entomopathogenic nematode commensal bacteria isolated from Sitrolla and Scelis. In order to clarify the antibacterial activity of these two kinds of commensal bacteria, their antibacterial activities against six major pathogenic fungi on apple were tested respectively. The results showed that the two kinds of commensal bacteria had different degrees of inhibition on six kinds of apple pathogenic fungi. The inhibitory rate of HBsAg strain HB310 was the best, with the inhibitory rate of 76.9% and the inhibitory effect on other pathogens as apple tree rot pathogen (74.1%)> apple spot defoliating pathogen 60.9%)> the pathogen of gray mold of apple (46.7%)> the pathogen of blue mold of apple (44.5%)> the pathogen of apple anthracnose (23.0%); the inhibitory effect of the broth of luminescent bacillus HB140 on the pathogen of gray mold of apple was the best , And the inhibition rate reached 86.7%. The pathogenic fungi showed the following effects: apple tree rot pathogen (76.6%)> apple rot pathogen (58.8%)> apple spot disease (46.0%)> (44.2%)> apple anthracnose pathogen (39.9%). The two kinds of commensal bacteria fermentation broth have strong inhibitory effect on the pathogen of apple rings, the EC50 were 42.28 and 42.92 mL / L respectively.