1975年Kohler和Milstein首先利用细胞融合技术,建立起制备杂交瘤单克隆抗体的有效方法。杂交瘤细胞系的建立,是近年来细胞生物学和免疫学中的一项重大突破。它不仅为制备血清制剂提供了一种新技术,而且也大大地提高了对许多疾病的诊断和治疗水平。随着这一技术的迅速发展,其应用范围亦日益广泛。近年来,单克隆抗体在乙型肝炎病毒中的应用亦有所进展,现仅将在这方面所开展的工作作一扼要的介绍。 In 1975, Kohler and Milstein first used cell fusion technology to establish an effective method for preparing monoclonal antibodies to hybridomas. The establishment of hybridoma cell lines is a major breakthrough in cell biology and immunology in recent years. It not only provides a new technology for preparing serum preparations, but also greatly improves the diagnosis and treatment of many diseases. With the rapid development of this technology, its application scope is also increasingly widespread. In recent years, the application of monoclonal antibodies in the hepatitis B virus has also made some progress. At present, only the work carried out in this respect is briefly introduced.