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应用支气管肺泡灌洗液进行细胞学检测时,标本制备方法对细胞定量有显著影响,许多研究表明细胞离心制片法导致淋巴细胞数量减少,而膜滤过法导致中性粒细胞数量减少,用检测末梢血时用的涂片技术制备支气管肺泡灌洗液标本是一简单的可用来代替离心制片法和膜滤过法进行标本制备及细胞分类计数的方法。 应用标准方法对35份肺泡灌洗液标本进行细胞分离,然后用血球计数器计数细胞,再比较涂片法与细胞离心制片所制备涂片的细胞分类。用200UL含4万个细胞的灌洗液通过细胞离心制片法(3分钟、57×g、Cytospin2)和用50UL内含5×10~5个细胞的灌洗液通过涂片法分别制备涂片,两种涂片均置空气中自然干
When using the bronchoalveolar lavage fluid for cytology, the specimen preparation method has a significant effect on the quantitation of the cells. Many studies have shown that the cytocentrifugal method leads to a decrease in the number of lymphocytes, whereas the membrane filtration method leads to a decrease in the number of neutrophils, Smear Technique for Detection of Peripheral Blood The preparation of bronchoalveolar lavage fluid specimens is a simple alternative to centrifugation and membrane filtration for specimen preparation and cell counting. 35 samples of bronchoalveolar lavage fluid were isolated by standard methods and then counted using a hemocytometer to compare the cell types of the smears prepared by smear and cell centrifugation. Cytospin2 was lysed with 200 L of a 40,000 cell-containing lavage fluid by a cytospinning method (3 minutes, 57 × g, Cytospin2) and a lavage fluid containing 5 × 10 -5 cells Film, both smears are set in the air dry naturally