目的:观察不同透析龄的腹膜透析（peritoneal dialysis，PD）患者腹膜组织中腹膜间皮细胞（human peritoneal mesothelial cells，HPMCs）及血管内皮细胞表达钠-葡萄糖协同转运蛋白（Nan +-dependent glucose transporter，SGLT）的差异，以及研究高糖处理对原代HPMCs内SGLT1、SGLT2蛋白表达的影响。n 方法:根据透析龄将PD患者分为4组：0年组、>0~2年组、>2~4年组和>4年组。采用HE及Masson染色观察PD患者腹膜组织形态学变化，免疫组化检测腹膜HPMCs及血管内皮细胞SGLT1、SGLT2的表达。从腹透液中提取并培养原代HPMCs，高糖、高渗培养液处理HPMCs 0 h、12 h、24 h、48 h、72 h及96 h，采用Western印迹法检测HPMCs的SGLT1、SGLT2蛋白含量，采用CCK-8试剂盒检测细胞活力。结果:HE及Masson染色显示，0年组PD患者壁层腹膜光滑连续，可见一层扁平HPMCs；>0~2年组PD患者HPMCs较0年组减少；>2~4年组PD患者HPMCs体积增大，数量减少；>4年组PD患者腹膜明显增厚、纤维化明显，HPMCs几乎不可见。免疫组化显示，随着透析龄增加，HPMCs内SGLT1、SGLT2蛋白表达逐渐下降（n P0.05）。用60 mmol/L葡萄糖处理原代HPMCs，可以先上调（0 h、12 h、24 h），后下调（24 h、48 h、72 h、96 h）SGLT1的表达（n P=0.029）；高糖处理对原代HPMCs中SGLT2的表达无明显影响。n 结论:高糖和透析龄的增加可导致HPMCs数量减少，活力下降，SGLT1、SGLT2表达下降，但对腹膜血管内皮细胞SGLT1、SGLT2的表达无明显影响。“,”Objective:To investigate the expression of Nan +-dependent glucose transporter(SGLT) in human peritoneal mesothelial cells (HPMCs) and vascular endothelial cells in peritoneal tissues of peritoneal dialysis (PD) patients at different dialysis vintages, and to study the influence of high glucose treatment on the expression of SGLT1 and SGLT2 in primary HPMCs.n Methods:According to the dialysis vintage, PD patients were divided into four groups: 0 year group, >0-2 years group, >2-4 years group and >4 years group. HE and Masson staining were used to observe the morphologic changes of peritoneal tissues in PD patients. Immunohistochemical staining was used to detect the expression of SGLT1 and SGLT2 in peritoneal HPMCs and vascular endothelial cells. The primary HPMCs were extracted from the peritoneal dialysis fluid, and treated with high-glucose or high-mannitol for 0 h, 12 h, 24 h, 48 h, 72 h and 96 h. Western blotting was used to investigate the SGLT1 and SGLT 2 expression in HPMCs. The cell viability was detected by using cell counting kit (CCK-8).Results:HE and Masson staining showed that the peritoneum of PD patients in 0 year group was smooth and continuous, with a flat layer of HPMCs. The number of HPMCs in>0-2 years group decreased compared with that in 0 year group. The HPMCs size increased in>2-4 years group, but the number decreased. The peritoneum of PD patients in>4 years group was significantly thickened and fibrotic, and HPMCs almost disappeared. Immunohistochemical staining showed that the expression of SGLT1 and SGLT2 in HPMCs gradually decreased with the increase of dialysis vintage (n P0.05). SGLT1 in primary HPMCs could be up-regulated (0 h, 12 h and 24 h), and then down-regulated (24 h, 48 h, 72 h, 96 h) with the treatment of 60 mmol/L glucose (n P=0.029); but there was no significant difference of SGLT2.n Conclusion:High glucose and the increase of dialysis vintage can reduce the number and the viability of HPMCs, and decrease the expression of SGLT1 and SGLT2, but there was no significant influence on SGLT1 and SGLT2 in peritoneal vascular endothelial cells.