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目的:研究双链蛋白聚糖(biglycan,BGN)对人胃癌细胞系SGC-7901的细胞增殖、周期及凋亡等生长作用影响。方法:构建重组质粒pIRES2-EGFP/BGN,转染胃癌细胞株SGC-7901,获得胃癌稳转细胞株SGC-7901/BGN。通过细胞增殖实验(CCK-8法)、平板克隆实验、流式细胞技术,分别检察BGN过表达对SGC-7901细胞增殖、克隆形成、周期及凋亡的影响。结果:SGC-7901/BGN组胃癌细胞较SGC-7901/空载组生长明显抑制(P<0.01),细胞培养板形成的克隆数明显减少[(209.7±12.6)比(326.0±15.1)];同时过表达BGN可促进胃癌细胞的凋亡[(10.7±1.5)%比(5.3±1.1)%],且将细胞周期阻止在G1期[(55.7±2.1)%比(37.6±1.8)%]。结论:胃癌细胞过表达BGN可抑制胃癌细胞的增殖、克隆形成。BGN可能通过将细胞周期阻滞在G1期、诱导细胞凋亡,从而发挥其生长抑制的生物学效应。
AIM: To investigate the effect of biglycan (BGN) on the proliferation, cell cycle and apoptosis of human gastric cancer cell line SGC-7901. Methods: The recombinant plasmid pIRES2-EGFP / BGN was constructed and transfected into gastric cancer cell line SGC-7901 to obtain the stable gastric cancer cell line SGC-7901 / BGN. The effects of BGN overexpression on proliferation, colony formation, cell cycle and apoptosis of SGC-7901 cells were examined by cell proliferation assay (CCK-8), plate clone assay and flow cytometry. Results: The growth of gastric cancer cells in SGC-7901 / BGN group was significantly inhibited (P <0.01) compared with that in SGC-7901 / empty vector group (209.7 ± 12.6 vs 326.0 ± 15.1) Meanwhile, overexpression of BGN could promote the apoptosis of gastric cancer cells [(10.7 ± 1.5)% vs (5.3 ± 1.1)%], and arrest the cell cycle in G1 phase [(55.7 ± 2.1)% vs (37.6 ± 1.8)%] . Conclusion: Overexpression of BGN in gastric cancer cells can inhibit the proliferation and colony formation of gastric cancer cells. BGN may exert the biological effects of its growth inhibition by blocking the cell cycle in G1 phase and inducing apoptosis.