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在水温15~18℃、pH 7.0±0.2、总硬度(以CaCO3计)380.00mg/L试验条件下,选用三氯异氰尿酸(0.75、1.14、1.73、2.63、4.00mg/L)、硫酸铜(0.80、1.50、2.82、5.31、10.00mg/L)、高锰酸钾(3.00、3.59、4.27、5.07、6.00mg/L)、溴氯海因(1.92、2.78、4.02、5.81、8.40mg/L)、聚维酮碘(13.00、17.53、23.66、31.92、43.00mg/L)5种常用药物对平均体质量6.99g、平均体长8.03cm的拉氏鱼种进行急性毒性试验。试验结果表明,5种药物24h半致死质量浓度分别为2.24、2.38、5.05、6.00、30.74mg/L;48h半致死质量浓度分别为1.43、2.18、4.31、4.08、23.33 mg/L;安全质量浓度分别为0.17、0.60、0.94、0.57、4.03mg/L。拉氏对5种药物的敏感性依次为:三氯异氰尿酸>溴氯海因>硫酸铜>高锰酸钾>聚维酮碘;聚维酮碘、溴氯海因、硫酸铜可安全使用。该研究为拉氏合理用药提供参考。
Under the conditions of water temperature of 15-18 ℃, pH 7.0 ± 0.2 and total hardness of 380.00mg / L as CaCO3, trichloroisocyanuric acid (0.75,1.14,1.73,2.63,4.00mg / L), copper sulfate (0.80,1.50,2.82,5.31,10.00mg / L), potassium permanganate (3.00,3.59,4.27,5.07,6.00mg / L), bromochlorothyrin (1.92,2.78,4.02,5.81,8.40mg / L, Povidone iodine (13.00,17.53,23.66,31.92,43.00mg / L) five kinds of commonly used drugs for the average body weight of 6.99g, an average body length of 8.03cm of Lasius catfish species acute toxicity test. The results showed that the median lethal concentrations of the five drugs were 2.24,2.38,5.05,6.00,30.74mg / L, respectively; the 48h median lethal concentrations were 1.43,2.18,4.31,4.08,23.33 mg / L, respectively. The safe mass concentration Respectively 0.17,0.60,0.94,0.57,4.03mg / L. The sensitivity of Lassie to five drugs was as follows: TCCB> BCC> copper sulfate> potassium permanganate> Povidone iodine; Povidone iodine, bromocholine and copper sulfate Safe to use. The study provides a reference for the rational use of drugs.