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目的探讨创伤对机体脂质过氧化及抗氧化能力的影响。方法选择普通级新西兰兔11只采集血样,采集后双侧耳缘小静脉反复穿刺,隔日1次,连续2—3次,建成创伤模型后采集血样,检测建模前后2组血清丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)指标,并予以统计分析。结果 MDA:A组(3.048±1.259)nmol/L,B组(3.251±1.695)nmol/L,A组水平低于B组;T-SOD:A组(384.314±50.122)U/m L,B组(348.942±45.852)U/m L,A组水平高于B组;CAT:A组(6.121±4.097)U/m L,B组(4.563±2.223)U/m L,A组水平高于B组;GSH-Px:A组(377.808±72.726)U/m L,B组(349.942±54.021)U/m L,A组水平高于B组;T-AOC:A组(5.537±2.128)U/m L,B组(4.633±2.074)U/m L,A组水平高于B组;组间差异均无统计学意义(P>0.05)。结论创伤对机体脂质过氧化及抗氧化能力具有一定影响,但无显著性影响。
Objective To investigate the effects of trauma on lipid peroxidation and antioxidant capacity in vivo. Methods Blood samples were collected from 11 New Zealand white rabbits. Blood samples were taken from the ventral marginal veins repeatedly and once or twice a day for 2-3 consecutive days. Blood samples were collected before and after the model was established. Serum malondialdehyde MDA, GSH-Px and T-AOC were measured and analyzed statistically. Results The level of MDA in group A was (3.048 ± 1.259) nmol / L, in group B was (3.251 ± 1.695) nmol / L, in group A was lower than in group B, and in group A was (384.314 ± 50.122) U / m L Group (348.942 ± 45.852) U / m L, the level of A group was higher than that of group B; the level of CAT in group A was (6.121 ± 4.097) U / m L and that in group B was 4.563 ± 2.223 U / B group; GSH-Px: A group (377.808 ± 72.726) U / m L, B group (349.942 ± 54.021) U / m L, A group was higher than B group; T-AOC: A group (5.537 ± 2.128) U / m L, B group (4.633 ± 2.074) U / m L, the level of A group was higher than that of B group. There was no significant difference between two groups (P> 0.05). Conclusion Trauma has some effects on the lipid peroxidation and antioxidant capacity of the body, but no significant effect.