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近年来,许多被认为在凋亡调节过程中具有重要作用的基因能在黄体中表达已被证实和鉴定。已知Bcl-2家族包括抑制凋亡(bc1-2,bc1-x_L,mc1-1和促进凋亡(bax)的成员在不同种属中均可表达。胱氨酸天冬氨酸特异的蛋白酶-3(Caspsse-3)是秀丽线虫(C.elegans)细胞死亡基因—ced-3—编码蛋白的类似物,其过度表达可导致真核细胞的凋亡。细胞凋亡可参与溶黄体过程,因此在黄体退化过程中具有一定作用,业已证明前列腺素F_(2α)(PGF_(2α))作为一种溶黄体因子可抑制黄体孕酮分泌。本研究旨在:①分离编码羊caspase-3的反向转录脱氧核糖核酸
In recent years, many genes that are considered to play an important role in apoptosis regulation can be expressed in the corpus luteum have been confirmed and identified. It is known that members of the Bcl-2 family, including apoptosis-inhibiting (bc1-2, bc1-x_L, mc1-1 and bax-promoting), are expressed in different species. Cystine-specific protease -3 (Caspsse-3) is an analogue of the gene -ced-3-encoded protein of the C. elegans cell death gene whose overexpression leads to the apoptosis of eukaryotic cells Apoptosis can participate in the process of levomerism, So it plays a role in the process of luteal degeneration.It has been shown that prostaglandin F_ (2α) (PGF_ (2α)) as a luteinizing factor can inhibit the secretion of progesterone in lutein.This study aimed to: (1) To isolate the gene encoding caspase-3 Reverse transcription of DNA