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目的探讨干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)对大鼠实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)的作用。方法 Wistar大鼠通过局部注射豚鼠全脊髓匀浆和完全弗氏佐剂制成的抗原乳剂诱导EAE模型,应用双抗夹心ELISA、放射免疫分析法分别检测实验大鼠血清IFN-γ和TNF-α的表达,并观察两种细胞因子的改变与EAE症状之间的关系。结果诱导EAE后,伴随着大鼠EAE症状的出现和进行性加重,其血清中IFN-γ和TNF-α表达较正常组分别升高了173.86%和166%。结论 IFN-γ和TNF-α在EAE的发生和发展密切相关,这两种细胞因子在EAE发病机制中的重要作用为探索新的有效治疗多发性硬化症(multiple sclerosis,MS)的手段提供了重要理论依据。
Objective To investigate the effects of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) on experimental autoimmune encephalomyelitis (EAE) in rats. Methods Wistar rats were induced by local injection of antigen emulsion of guinea pig full spinal cord homogenate and complete Freund’s adjuvant. The levels of IFN-γ and TNF-α in serum of experimental Wistar rats were detected by double-antibody sandwich ELISA and radioimmunoassay , And observed the relationship between changes in both cytokines and EAE symptoms. Results After induction of EAE, the expression of IFN-γ and TNF-α in serum increased by 173.86% and 166%, respectively, with the onset and progressive increase of EAE in rats. Conclusions IFN-γ and TNF-α are closely related to the occurrence and development of EAE. The important role of these two cytokines in the pathogenesis of EAE may provide a new means to effectively treat multiple sclerosis (MS) Important theoretical basis.