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心衰康冲剂是以红参、附子、川芎、葶苈子等制成的中成药,为了研究其对心肌细胞代谢的影响,探讨其可能的作用机理,特进行如下实验。1 材料1.1 主要仪器 LXJ—Ⅱ离心机(上海医用分析仪器J);HH·W_(21)·Cr600型电热恒温水温箱(北京长安科学仪器厂);721—光度分析仪(上海分析仪器三厂)。1.2 主要药品及试剂 心衰康(河南中医学院一附院制剂室提供,批号931013);注射用盐酸阿霉素(ADR)(汕头鮀滨制药厂,批号930221),Na_2ATP(上海华美生物工程公司进口分装,编号H1201);牛血清白蛋白(郑州博赛生物试剂所提供,批号
Heart failure Kang granules are proprietary Chinese medicines made from red ginseng, aconite, rhizoma chuanxiong, wolfberry fruit, etc. In order to study its effect on myocardial cell metabolism and to explore its possible mechanism of action, the following experiments are particularly conducted. 1 Materials 1.1 Major Instruments LXJ-II Centrifuge (Shanghai Medical Analytical Instrument J); HH·W_(21)·Cr600 Type Electric Thermostatic Water Thermostat (Beijing Chang’an Scientific Instruments Factory); 721—Photometric Analyzer (Shanghai Analytical Instrument Factory) ). 1.2 Major Drugs and Reagents Xinshuikang (Provided by the Preparation Department of the First Affiliated Hospital of Henan College of Traditional Chinese Medicine, Lot 931013); Adriamycin Hydrochloride (ADR) for Injection (Shantou Weibin Pharmaceutical Factory, Lot 930221), Na_2ATP (Shanghai Huamei Bioengineering Co., Ltd.) Imported subpackaged, number H1201); bovine serum albumin (provided by Zhengzhou Bosai Biological Reagent, batch number