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目的:观察外源性肾上腺髓质素(AM)对卵巢癌细胞HO8910迁移的影响,并探讨其作用机制。方法:外源性应用AM后,采用划痕实验观察卵巢癌细胞系HO8910的迁移功能,并且利用蛋白质印迹法检测ERK1/2及p-ERK1/2蛋白的表达,以了解AM对ERK1/2蛋白及其活性的影响。结果:外源性给予AM(100nmol/L)的细胞12h迁移率为(62.61±4.51)%,阴性对照细胞的迁移率为(29.23±4.15)%,AM可以促进卵巢癌细胞HO8910的迁移,P=0.001。提前应用ERK1/2抑制剂PD98059的细胞在外源性AM(100nmol/L)的刺激下迁移率为(37.97±3.44)%,比单纯应用AM刺激的细胞迁移率降低,P=0.002。外源性给予AM(100nmol/L)可以促进卵巢癌细胞的ERK1/2蛋白磷酸化。结论:AM促进卵巢癌细胞的迁移,与细胞的ERK1/2活化相关,可能为卵巢癌迁移的治疗提出一个新的研究方向。
Objective: To observe the effect of exogenous adrenomedullin (AM) on the migration of ovarian cancer cell line HO8910 and to explore its mechanism. Methods: Migration assay of ovarian cancer cell line HO8910 was performed using scratch assay after exogenous administration of AM, and the expression of ERK1 / 2 and p-ERK1 / 2 protein was detected by Western blotting to understand the effect of AM on ERK1 / 2 protein And its activity. RESULTS: The 12h cell migration rate was (62.61 ± 4.51)%, while the negative control cell migration rate was (29.23 ± 4.15)%. AM promoted the migration of HO8910 in ovarian cancer cell line P (100nmol / L) = 0.001. The cell migration rate of ERK1 / 2 PD98059 stimulated by exogenous AM (100 nmol / L) was (37.97 ± 3.44)%, which was lower than that of cells stimulated by AM alone (P = 0.002). Exogenous administration of AM (100 nmol / L) can promote ERK1 / 2 phosphorylation in ovarian cancer cells. Conclusion: AM can promote the migration of ovarian cancer cells, which is related to the activation of ERK1 / 2 in cells and may provide a new research direction for the treatment of ovarian cancer.