We report the largest single-center experience with robotic-assisted microscopic varicocelectomy (RAMV) in male infertility. From August 2012 to February 2015, men with infertility of at least a year and varicoceles underwent RAMV by a single surgeon. Varicocele was diagnosed on physical examination and confirmed by ultrasound by a single ultrasonographer. Preoperative hormone panel, semen analyses, and testicular Doppler ultrasound were obtained from all men and repeated at 3 months. One hundred and forty consecutive men (258 varicocelectomies) were included. Mean age and duration of infertility was 36.4 and 2.8 years, respectively. Median total and free testosterone increased by 145 ng dl?1 and 4.3 pcg ml?1 (44.3％), respectively (P < 0.0001). Median sperm concentration increased by 37.3％ (P < 0.03). Median sperm motility and morphology did not significantly change. Median left and right testicular volume increased by 22.3％ (P < 0.0001) and 12.6％ (P < 0.0006), respectively. Hydroceles occurred 0.8％ of procedures. We had no testicular artery injuries. Persistence of varicocele by Doppler ultrasound was 9.6％. Only 37.3％ of patients required pain medications postoperatively. We concluded that RAMV is a safe and effective alternative for varicocele repair with outcomes comparable to historical traditional microsurgical approach.