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目的 探讨房颤时心房肌细胞膜上T型Ca2 + 通道对肌浆网上IP3 R和RyR表达及功能的影响。方法 杂种犬 15条 ,随机分为正常对照组、单纯房颤组和房颤 +Mibefradil组。房颤组用起搏器进行右心房快速起搏(5 2 0 /min)。房颤 +Mibefradil组在起搏术后第 2天开始给mibefradil。正常对照组不植入起搏器。胶原酶Ⅱ型分离心房肌细胞 ,用激光共聚焦显微镜检测T型Ca2 + 通道阻滞剂对肌浆网上三磷酸肌醇受体 (IP3 R)和兰尼碱受体(RyR)表达及功能的影响。结果 房颤 +Mibefradil组的RyR/IP3 比值 (0 2 96 5± 0 0 812 )比正常对照组 (2 70 4 3±0 2 2 93)明显减少 (P <0 0 5 ) ,而与单纯房颤组 (0 2 4 72± 0 135 5 )比较无统计学意义 ;房颤 +Mibefradil组的心房肌细胞在给予caffeine刺激时细胞内Ca2 + 浓度增高不明显 (1 30 31± 0 10 5 6 ) ,与正常对照组 (1 74 10± 0 15 6 6 )比较有统计学意义 (P <0 0 5 ) ,而与单纯房颤组 (1 2 5 6 1± 0 0 6 4 6 )比较无统计意义 ;房颤 +Mibefradil组的心房肌细胞在给予ATP刺激时细胞内Ca2 + 浓度增高不明显 (1 186 6± 0 6 6 91) ,与正常对照组 (1 2 319± 0 2 2 73)比较无统计学意义 ,而比单纯房颤组 (2 2 90 4± 0 6 4 71)明显减少 (P <0 0 5 )。结论 房
Objective To investigate the effect of T-type Ca2 + channels on the expression and function of IP3R and RyR on sarcoplasmic reticulum in atrial fibrillation. Methods Fifteen hybrid dogs were randomly divided into normal control group, atrial fibrillation group and atrial fibrillation + Mibefradil group. Atrial fibrillation group pacemaker right atrial pacing (520 / min). Atrial fibrillation + Mibefradil group began giving mibefradil on day 2 after pacing. Normal control group did not implanted pacemaker. Collagenase Ⅱ was used to separate atrial myocytes. Laser confocal microscopy was used to detect the expression and function of T-type Ca2 + channel blockers on sphingomyelin receptor (IP3R) and ryanodine receptor (RyR) influences. Results The RyR / IP3 ratio (0 2 96 5 ± 0 0 812) in atrial fibrillation + Mibefradil group was significantly lower than that in the normal control group (2 704 3 ± 0 2 2 93) (P 0 05) Atrial fibrillation + Mibefradil group of atrial myocytes in Caffeine-stimulated intracellular Ca2 + concentration was not significantly increased (13031 ± 0 1056) compared with no statistically significant , Compared with the normal control group (1 74 10 ± 0 15 6 6) (P 0 05), but no statistical difference compared with the atrial fibrillation group (1 25 6 1 ± 0 0 6 46) Significance of atrial fibrillation + Mibefradil group of atrial myocytes in the stimulation of ATP intracellular Ca2 + concentration was not significantly increased (1 186 6 ± 0 6 6 91), compared with the normal control group (12 319 ± 0 2 2 73) No statistical significance, but significantly lower than that in the atrial fibrillation group (2 2 90 4 ± 0 6 4 71) (P 0 05). Conclusion room