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目的对婴幼儿喘息患者鼻咽分泌物涂片中嗜酸性粒细胞计数的四种染色方法进行比较。方法62例喘息患儿各取鼻咽分泌物1ml,制成4张涂片。经Carnoy氏液固定后,分别采用HE、Wright、WrightGiemsa及“1min染色”4种方法进行染色、读片和比较。结果4种染色检出嗜酸性粒细胞的阳性率分别为77.4%、79.0%、73.8%和75.8%。结论Wright或WrightGiemsa染色的方法较简便,显色清楚,可作为诊断哮喘的辅助手段。
Objective To compare the four staining methods of eosinophil count in nasopharyngeal secretions in infants and young children with asthma. Methods 62 cases of asthmatic children take nasopharyngeal secretions 1ml, made of 4 smears. After fixation with Carnoy’s solution, the four methods of HE, Wright, Wright Giemsa and “1 min staining” were used for staining, reading and comparing. Results The positive rates of eosinophils detected by four kinds of staining were 77.4%, 79.0%, 73.8% and 75.8% respectively. Conclusion Wright or Wright Giemsa staining method is simple, clear color, can be used as an adjunct to diagnosis of asthma.