论文部分内容阅读
女性怀孕前后饮酒会对胎儿的发育及神经系统造成不利影响,称为“胎儿酒精综合征”(fetal alcohol spectrum disorders,FASD)。小鼠通常作为研究该病的动物模型。该实验采用体外培养技术及体内冲胚法研究雌鼠怀孕前后酒精摄入对各期植入前胚胎全基因组DNA甲基化模式建立的影响。小鼠植入前胚胎体外培养实验发现,体外实验组I(怀孕前酒精处理组),除8-cell外,其他各期胚胎的DNA甲基化水平明显低于体外对照组;体外实验组II(正常胚胎在含乙醇的培养基中培养),各期植入前胚胎DNA甲基化水平均明显低于体外对照组。体内实验发现,体内实验组I(怀孕前酒精处理组)与体内的实验组II(怀孕后酒精处理组),各期植入前胚胎DNA甲基化水平明显低于体内对照组。体内、外实验结果表明:受精前后酒精对各期植入前胚胎DNA甲基化模式的正确建立造成紊乱,该结果可为进一步揭示FSAD发病机制提供一定的实验基础。
Pre and post-pregnancy alcohol consumption in women adversely affects the development of the fetus and the nervous system, known as “fetal alcohol spectrum disorders” (FASD). Mice are commonly used as animal models to study the disease. In this study, we used in vitro culture techniques and in vivo embryos to study the effects of alcohol intake before and after pregnancy on methylation patterns of whole genome DNA of preimplantation embryos. In vitro experiments of mouse preimplantation embryos showed that in vitro group I (pretreatment alcohol treatment group), except for 8-cell, DNA methylation levels in other embryos were significantly lower than those in control group. In vitro experiment group II (Normal embryos were cultured in ethanol-containing medium). The DNA methylation levels of preimplantation embryos in each stage were significantly lower than those in the control group. In vivo experiments showed that in vivo experimental group I (pre-pregnancy alcohol treatment group) and experimental group II (post-pregnancy alcohol treatment group), the pre-implantation embryo DNA methylation levels were significantly lower than the control group. The results of in vitro and in vivo experiments showed that alcohol pre-implantation and post-implantation pre-implantation DNA methylation patterns of the correct establishment of disorder caused by the results of this study can further reveal the pathogenesis of FSAD to provide some experimental basis.