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伯氏疟原虫整个红外期循环的体外培养已获成功。作者用间接荧光抗体试验(IFAT)观察了整个红外期的发育过程。作者将感染有伯氏疟原虫的阳性斯氏按蚊,在无菌条件下解剖涎腺置于WI38的人胚胎肺的融合细胞培养物培养,每一培养基中放入4对无菌的涎腺。接种后0.5、1、3、6、8、10、12、24和48小时用吉氏液染色和间接荧光抗体试验。间接荧光抗体试验使用对子孢子有反应而对红细胞期原虫无反应的鼠抗
In vitro culture of P. berghei throughout the IR cycle has been successful. The authors used an indirect fluorescent antibody test (IFAT) to observe the development of the entire IR period. The authors will be infected with Plasmodium berghei-positive An. Anopheles, dissecting the salivary glands under aseptic conditions in a human embryonal lung WI38 fused cell culture culture, each containing 4 pairs of sterile salivary glands gland. 0.5, 1, 3, 6, 8, 10, 12, 24 and 48 hours after inoculation with Git staining and indirect fluorescent antibody test. Indirect fluorescent antibody test uses a murine antibody that responds to sporozoites and does not respond to erythrocytic protozoa