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目的:研究葡萄籽原花青素对大鼠脑缺血再灌注损伤的保护作用。方法:采用尼龙线栓塞大鼠大脑中动脉制备大鼠局灶性脑缺血再灌注模型,观察不同剂量的葡萄籽原花青素对脑梗死面积、脑组织含水量及血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)活性的影响。结果:葡萄籽原花青素3、15、30 mg·kg-1可显著减小脑缺血再灌注损伤大鼠的行为评分,减轻缺血侧脑半球水肿程度,缩小脑梗死面积,显著降低血清中CK、LDH的活性,明显升高血清中SOD的活性。结论:葡萄籽原花青素对大鼠脑缺血再灌注损伤有保护作用,其升高血清SOD活性作用可能是其保护作用的机制之一。
Objective: To study the protective effect of grape seed procyanidins on cerebral ischemia-reperfusion injury in rats. METHODS: A rat model of focal cerebral ischemia and reperfusion was prepared by embolizing the middle cerebral artery of the rat using nylon thread. The infarct size, brain tissue water content, and creatine kinase (CK) in the serum were observed at different doses of grape seed procyanidins. Effects of lactate dehydrogenase (LDH) and superoxide dismutase (SOD) activity. RESULTS: Grape seed procyanidins 3, 15 and 30 mg·kg-1 significantly reduced the behavioral scores of rats with cerebral ischemia-reperfusion injury, reduced the cerebral hemisphere edema in the ischemic hemisphere, and reduced the infarct size, and significantly reduced the serum CK level. The activity of LDH significantly increased the activity of SOD in serum. Conclusion: Grape seed procyanidins have a protective effect on cerebral ischemia-reperfusion injury in rats. Increasing serum SOD activity may be one of the protective mechanisms.