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目的观察转化生长因子６（ＴＧＦ－β）对培养的人角膜基质细胞分泌ＭＭＰ－２和ＭＭＰ－９的调节作用，探讨ＴＧＦ－β治疗非感染性角膜溃疡的潜在可能性。方法采用酶谱法检测人角膜基质细胞ＭＭＰ－２和ＭＭＰ－９的分泌，并观察 ＴＧＴ－β对 ＭＭＰ－２和 ＭＭＰ－９分泌的调节作用。结果培养的人角膜基质细胞分泌 ＭＭＰ－２和 ＭＭＰ－９，ＴＧＦ－β抑制ＭＭＰ－９的活性，且随ＴＧＦ－β质量浓度增加而增强，随ＴＧＦ－β作用时间延长而增强；对ＭＭＰ－２的活性，ＴＧＦ－β有较弱的促进作用。结论ＴＧＦ－β作为一种ＭＭＰ－９的抑制剂，在促进非感染性角膜溃疡愈合方面很有潜力。
Objective To investigate the regulatory effect of transforming growth factor-6 (TGF-β) on secretion of MMP-2 and MMP-9 by cultured human corneal stromal cells and to explore the potential of TGF-β in the treatment of non-infected corneal ulcer. Methods The secretion of MMP-2 and MMP-9 in human corneal stroma cells was detected by zymography, and the regulation of the secretion of MMP-2 and MMP-9 by TGT-β was observed. Results The cultured human corneal stromal cells secreted MMP-2 and MMP-9, the activity of TGF-β inhibited the activity of MMP-9 and increased with the increase of TGF-β concentration, 2 activity, TGF-β has a weaker role in promoting. Conclusion TGF-β, as a MMP-9 inhibitor, has great potential in promoting the healing of non-infectious corneal ulcer.