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以华盛顿脐橙（Ｃｉｔｒｕｓｓｉｎｅｎｓｉｓ）离体春梢１月龄叶片为试材，在控光（０．５μｍｏｌ／ｍ２ｓ及控温（３０±１）℃条件下进行２４ｈ５０ｍｍｏｌ／ＬＮＨ４Ｃｌ饲喂处理并对相关代谢产物进行了测定．结果表明，叶片ＮＨ３－ＮＨ＋４、游离精氨酸及腐胺含量均直线上升，且呈平行变化；对精氨酸生物合成强度及精氨酸脱羧酶活性的１４Ｃ示踪测定证实了二者的加强是导致精氨酸和腐胺含量增加的主要原因；进一步的直线回归分析显示，上述代谢产物之间及其与精氨酸生物合成强度和精氨酸脱羧酶活性之间存在密切的关联．
The leaves of 1-month-old spring shoots of Citrus sinensis in Washington DC were used as materials to conduct 24h50mmol / LNH4Cl feeding under light control (0.5μmol / m2s and temperature control (30 ± 1) ℃) The results showed that the contents of NH3-NH + 4, free arginine and putrescine increased linearly and varied in parallel. The 14C tracing of arginine biosynthesis intensity and arginine decarboxylase activity was confirmed Both enhancements are responsible for the increased arginine and putrescine levels; further linear regression analysis revealed a close relationship between the above metabolites and their relationship with arginine biosynthesis and arginine decarboxylase activity The association.