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采用不同浓度(0～60mg/L)的氯化钕(neodymium chloride)对水稻品种进行液体培养,获得线粒体。分析了水稻线粒体抗氧化保护酶及呼吸代谢酶和能量转换酶(COX、SOD、GSH-Px、Ca2+-ATPase、Mg2+-ATPase)活性的变化。结果表明:适当浓度(10～40mg/L)的氯化钕对酶的活力具有明显的诱导和促进作用;但当稀土氯化钕的浓度升高到40～60mg/L时则对酶活力开始产生钝化和抑制作用。分析认为,线粒体抗氧化保护酶及呼吸代谢酶和能量转换酶活性的升高和增强,表明稀土钕可能通过提高线粒体酶的活力,而具有清除含氧自由基和提高作物产品质量的作用。
The rice varieties were cultured in liquid medium with neodymium chloride at different concentrations (0 ~ 60mg / L) to obtain mitochondria. The changes of mitochondrial antioxidant enzymes, respiratory metabolic enzymes and energy-converting enzymes (COX, SOD, GSH-Px, Ca2 + -ATPase, Mg2 + -ATPase) in rice were analyzed. The results showed that neodymium chloride with proper concentration (10 ~ 40 mg / L) could obviously induce and promote the enzyme activity. However, when the concentration of rare earth neodymium chloride increased to 40 ~ 60 mg / L, Produce passivation and inhibition. It is suggested that the increase and enhancement of mitochondrial antioxidant enzymes, respiratory metabolic enzymes and energy conversion enzyme activities indicate that rare earth neodymium may have the effect of scavenging oxygen free radicals and improving the quality of crop products by increasing mitochondrial enzyme activity.