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笔者应用分子生物学手段（ＰＣＲ）对该院１９９５～１９９７年门诊和住院的１３１例乙肝标志物不典型阳性患者进行ＨＢＶ－ＤＮＡ检测，以判断ＨＢＶ复制情况，结果报告如下。１ 材料与方法１．１ 材料：ＰＣＲ扩增仪和试剂均由华美生物工程公司提供。乙肝血清学标志物检测
The author used molecular biological means (PCR) of the hospital from 1995 to 1997 outpatient and inpatient 131 cases of HBV markers in patients with atypical positive HBV-DNA testing to determine HBV replication, the results are reported as follows. 1 Materials and Methods 1.1 Materials: PCR amplification instrument and reagents are provided by China and the United States bio-engineering company. Hepatitis B serological marker test