目的　观察谷氨酰胺在小肠保存中的作用。方法　 1 2只杂种猪随机分成 2组 ,供肠分别采用含有 2 2 g/L甘氨酸 (对照组 ,n =6)或 2 0 g/L谷氨酰胺 (实验组 ,n =6)的Euro Collins液保存2 4h ,然后行自体节段小肠移植 ,测定保存后及再灌注 30min时肠粘膜损伤Parks评分、肠粘膜谷氨酰胺含量、双糖酶活性、门静脉血中内毒素浓度和移植小肠的通透性。结果　保存 2 4h ,肠粘膜谷氨酰胺含量明显高于对照组 ;再灌注后 30min ,实验组肠粘膜的损伤明显轻于对照组 ,双糖酶活性明显高于对照组 ,而门静脉血中内毒素浓度明显低于对照组 ;两个组移植术后 2 4h肠粘膜通透性均明显增加 ,但对照组较实验组增加更明显。结论　保存液中添加谷氨酰胺能有效地改善移植小肠的功能 ,降低肠粘膜的通透性 ,减轻肠粘膜的组织损伤程度 Objective To observe the role of glutamine in the preservation of small intestine. Methods A total of 2 hybrid pigs were randomly divided into two groups, fed with Euro Collins containing 2 2 g / L glycine (control group, n = 6) or 20 g / L glutamine (experimental group, n = 6) Fluid preservation 24 h, and then the autologous segment small bowel transplantation measured after storage and reperfusion 30min intestinal damage Parks score, gut mucosal glutamine content, disaccharide activity, portal vein blood endotoxin concentration and transplantation of small intestine through Permeability. Results The content of glutamine in intestinal mucosa was significantly higher than that of the control group after 24 h of storage. The damage of intestinal mucosa in the experimental group was significantly lighter than that of the control group 30 min after reperfusion, and the activity of disaccharidase was significantly higher than that of the control group The concentration was significantly lower than the control group; two groups after transplantation 24 h intestinal mucosal permeability were significantly increased, but the control group increased more significantly than the experimental group. Conclusion The addition of glutamine in the preservation solution can effectively improve the function of the transplanted small intestine, reduce the permeability of the intestinal mucosa and reduce the tissue damage of the intestinal mucosa