论文部分内容阅读
目的研究人参皂苷元衍生物及其抗HL-60肿瘤细胞活性。方法利用Smith降解处理人参总皂苷,采用硅胶柱色谱、制备型高效液相色谱技术分离、纯化人参皂苷元等产物。通过波谱数据分析产物结构。采用MTT法测试产物抗HL-60肿瘤细胞活性。结果分离鉴定了原人参二醇(PD)、原人参三醇(PT)和24,25-烯-3β,6α-二羟基-12,20-(1′-羟基)双氧乙基-达玛烷(1),化合物1为新化合物命名为1′-羟基双氧乙基原人参三醇。结论化合物1为新型人参皂苷元衍生物,其抑制HL-60细胞增殖的作用强于PD和PT。
Objective To study ginsenoside derivatives and their anti-HL-60 tumor cell activity. Methods The total ginsenosides were treated with Smith degradation, and the products of ginsenosides were separated and purified by preparative high performance liquid chromatography with silica gel column chromatography. Product structure was analyzed by spectral data. The product was tested for anti-HL-60 tumor cell activity using MTT assay. RESULTS: Protopanaxadiol (PD), protopanaxatriol (PT) and 24,25-ene-3β, 6α-dihydroxy-12,20- (1’-hydroxy) Alkane (1), Compound 1 is a new compound named as 1’-hydroxy-dioxygen protopanaxol. Conclusion Compound 1 is a novel derivative of ginsenoside that inhibits the proliferation of HL-60 cells more strongly than PD and PT.