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用离子交换色谱和凝胶过滤技术从小牛胸腺中提取、纯化亲环素 Ａ（ＣｙＰ Ａ）。 ＳＤＳ－ＰＡＧＥ分析为单一条带，分子量为 １７．８ＫＤ，与 ＣｙＰ Ａ标准品一致。用此 ＣｙＰ Ａ建立了检测抗 ＣｙＰＡ自身抗体（ＡＣＡ）的ＥＬＩＳＡ法。在免疫豚鼠获得抗血清基础上，又建工了ＥＬＩＳＡ法检测ＣｙＰＡ特异免疫复合物（ＣｙＰ Ａ－ＩＣ）。正常对照（ｎ＝１００） ＡＣＡ和 ＣｙＰ Ａ－ＩＣ阳性率均仅 １．０％． ＳＬＥ患者（ｎ＝１０１） ＡＣＡ阳性率为 ５０．５％， ＣｙＰ Ａ－ＩＣ为 ７４．２％。结果提示，血清 ＡＣＡ和 ＣｙＰ Ａ－ＩＣ测定对ＳＬＥ的诊断有较高的敏感性和特异性。
Cyclophilin A (CyP A) was extracted from calf thymus by ion exchange chromatography and gel filtration. SDS-PAGE analysis of a single band, molecular weight of 17.8KD, consistent with the standard CyP A. An ELISA for detecting anti-CyPA autoantibodies (ACA) was established with this CyP A. On the basis of obtaining antiserum from guinea pigs, CyPA-specific immune complex (CyP A-IC) was also detected by ELISA. Normal controls (n = 100) both ACA and CyP A-IC positive rates were only 1.0%. In SLE patients (n = 101), the positive rate of ACA was 50.5% and that of CyP A-IC was 74.2%. The results suggest that serum ACA and CyP A-IC determination of the diagnosis of SLE have a higher sensitivity and specificity.