论文部分内容阅读
目的 :研究、探讨多向药耐药基因 (MDRl)及其表达产物P -糖蛋白 (P -gp)、拓扑异构酶Ⅱ (TopoⅡ )、谷胱甘肽 -S -转移酶 (GST -л)和胸苷酸合成酶 (Ts)的表达及其临床意义。方法 :采用SP法对 1 30例随访 1 0年以上的结、直肠患者进行了癌组织中P - gp、GST -л、TopoⅡ和TS的表达 ,并分析与临床预后的关系。结果 :GST -л的表达与UICC分期有关 (P =0 0 2 2 ) ,而与组织类型、浸润深度及淋巴结转移无关 ;P -gp、TopoⅡ和TS的表达与上述参数均无关 (P >0 0 5)。经多因素Cox比例风险模型分析显示 ,UICC分期、组织类型及GST -л、TopoⅡ的表达与结直肠癌患者术后生存率有关 (P <0 0 5)。结论 :检测结直肠癌中P -gp、GST -x、TopoⅡ和TS的表达 ,对肿瘤化疗药物的选择具有重要临床意义 ;UICC分期、组织类型及GST -л、TopoⅡ的表达似可作为结直肠癌患者独立的预后因素。
AIM: To investigate the expression of multidrug resistance genes (MDR1), P-glycoprotein (P-gp), topoisomerase Ⅱ (TopoⅡ) and glutathione S-transferase ) And thymidylate synthase (Ts) expression and its clinical significance. Methods: SP method was used to detect the expression of P - gp, GST - L, Topo Ⅱ and TS in 130 rectal cancer patients with over 100 years of follow - up. Results: The expression of GST-L was correlated with UICC staging (P = 0.0022), but not with histological type, depth of invasion and lymph node metastasis. The expressions of P-gp, TopoⅡ and TS were not related with the above parameters 0 5). The multivariate Cox proportional hazards model analysis showed that the UICC staging, tissue type, GST-л, Topo ¢ òexpression correlated with postoperative survival rate in patients with colorectal cancer (P <0.05). Conclusion: The detection of the expression of P-gp, GST-x, TopoⅡ and TS in colorectal cancer has important clinical significance for the choice of tumor chemotherapeutic drugs. The expression of UICC staging, tissue type and GST-л, TopoⅡ may be used as colorectal Cancer patients with independent prognostic factors.