The produced oils in central Junggar Basin are commonly mixed in origin. In this paper, in order to reveal this complexity and thereby provide valuable clues to the study of oil source and formation mechanism, genetic groups of the mixed oils were classified and their migration/accumulation was investigated. Based on the artificial oil mixing experiments, some representative biomarkers of the mixed oils showed varying tendencies according to mixing ratios of the oils. Hence, these biomarkers are useful for determining the origin of the mixed oils. According to the criteria, oils in the area were divided into four basic groups, i.e., the Lower Permian Fengcheng oil, the Middle Permian Lower Wuerhe oil, the Jurassic source derived oil, and the mixed oil (including the Lower and Middle Permian mixed oil and the Permian and Jurassic mixed oil). Oil migration and accumulation were discussed in combination with the geological background.