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对烟草幼苗叶面喷施不同浓度 ( 0 ,50 ,10 0 ,30 0 ,50 0 ,70 0 ,10 0 0 mg· L-1)的氯化胆碱 ( CC)溶液 ,研究 CC对其在经受 3～ 4℃冷胁迫下的保护性效果。结果表明 :与对照的比较 ,经 CC处理的烟草幼苗 ,在冷胁迫下 ,其超氧物歧化酶、过氧化氢酶和过氧化物酶等活性明显较高 ,故其膜脂过氧化产物丙二醛产生量减少 ,电解质外渗率降低 ,叶绿素残存量较高 ;经 CC处理的烟草幼苗总含水量与对照的差异虽然不大 ,但其束缚水含量增高 ,自由水减少 ,原因是体内可溶性糖、可溶性蛋白质和脯氯酸含量明显增高。试验结果表明在 0℃以上冷胁迫下 ,CC对膜脂有保护作用 ,并增加了细胞内抗冷性物质 ,有利于烟草抗冷性的提高。在不同浓度的 CC处理中 ,以喷施 30 0 mg· L-1CC者保护效果最佳
Choline chloride (CC) solution with different concentrations (0, 50, 10 0, 30 0, 50 0, 70 0, 100 mg · L -1) was sprayed on the leaves of tobacco seedlings Under 3 ~ 4 ℃ cold stress under the protective effect. The results showed that compared with the control, under CC stress, the activities of superoxide dismutase, catalase and peroxidase were significantly higher under cold stress, The amount of dialdehyde decreased, the electrolyte leakage rate decreased, and the chlorophyll content remained high. Although the difference between the total water content of CC seedlings treated with CC and the control was not significant, the bound water content increased and the free water decreased due to the in vivo solubility Sugar, soluble protein and proline content increased significantly. The results showed that under the cold stress of 0 ℃, CC could protect the membrane lipids and increase the intracellular cold-tolerance substances, which was in favor of improving the chilling resistance of tobacco. In different concentrations of CC treatment, spraying 30 0 mg · L-1CC best protection