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目的:采用复合造模法建立绝经期糖尿病骨质疏松症大鼠模型,从生物力学角度探讨双黄益骨方的防治作用、量效关系及作用机制。方法:将210只6个月龄雌性清洁级SD大鼠随机分为假手术组、模型组、预防组和治疗组(包括高剂量中药、低剂量中药组)并与尼尔雌醇组、立普妥组对照。除假手术组外,均行卵巢摘除术及注射链脲佐菌素诱导绝经期糖尿病骨质疏松症。分别于术后第10,24周取各组大鼠左股骨和L4行最大弯曲力、最大压缩力和最大凹入应力检测。～5结果:第24周假手术组的最大压缩力307.905±51.057)N犤(和最大凹入应力犤(70.180±15.269)N犦显著大于第10周假手术组犤(234.064±35.390),(50.986±9.925)N犦(P<0.05),而模型组第24周与第10周比较则呈下降趋势;模型组、中药预防组最大凹入应力较假手术组明显减低(P<0.05);第24周模型组最大压缩力、最大凹入应力分别为(犤137.014±72.560),(22.096±17.350)N较假手术组显著犦降低(P<0.001);高剂量中药组与尼尔雌醇组较第24周模型组最大压缩力显著提高犤分别为(212.698±54.661),(256.118±85.429)NP<0.05～0.01)。低剂量中药组与立普妥组在改善骨力学方面犦(未能显示出显著疗效。结论:正常组骨强度和变形力随月龄增长而提高,模型组则呈下降趋势,成模后,模型组较正常?
OBJECTIVE: To establish a rat model of menopausal osteoporosis with complex modeling and to explore the preventive and therapeutic effects, dose-effect relationship and mechanism of action of Shuanghuang Yigufang from a biomechanical point of view. METHODS: A total of 210 6-month-old female SD rats were randomly divided into sham-operated group, model group, prevention group, and treatment group (including high-dose traditional Chinese medicine and low-dose Chinese herbal medicine group) and were treated with nylestriol. Putuo group control. Except for the sham operation group, ovariectomy and injection of streptozotocin induced menopausal osteoporosis. At 10th and 24th weeks after operation, the maximum bending force, maximum compressive force and maximum reentrant stress of the left femur and L4 were measured. ～5 results: The maximum compressive force of the sham-operated group at week 24 was 307.905±51.057)N犤 (and the maximum reentrant stress 犤(70.180±15.269)N犦 was significantly greater than that of the sham-operated group at week 10 (234.064±35.390), ( (50.986±9.925)N犦(P<0.05), but decreased in the 24th and 10th weeks of the model group; the maximum concave stress in the model group and the Chinese medicine preventive group was significantly lower than that in the sham operation group (P<0.05). At week 24, the maximum compressive force and maximum recumbent stress in the model group were (犤137.014±72.560), and (22.096±17.350)N was significantly lower than that in the sham group (P<0.001); the high-dose Chinese medicine group was associated with nilestriol. Compared with the 24th week, the maximum compressive force of the model group was significantly increased (212.698±54.661), (256.118±85.429) NP<0.05-0.01. The low-dose Chinese medicine group and Lipitor group showed improvement in bone mechanics (failure to show significant efficacy. Conclusion: The bone strength and deformability in the normal group increased with the age of the month, and the model group showed a decreasing trend. Model group is more normal?