论文部分内容阅读
背景与目的白色念珠菌是常见的条件致病菌,定植于人体胃肠道和泌尿生殖道,可引起黏膜和皮肤的浅表感染。感染与否取决于增强的白色念珠菌毒力和受损的宿主防御系统之间的失衡。近来研究发现光动力治疗(photodynamic therapy,PDT)可用于抗微生物感染。本研究评价了亚甲蓝(0.05 m M)介导的亚致死量的PDT改变白念珠菌的致病性的能力,并观察了子代细胞是否维持这种特征的改变。材料和方法选取白念珠菌标准株ATCC90028为实验对象,50μM亚甲蓝为光敏剂,实验光源为Ga Al As二极管
Background and Objective Candida albicans is a common opportunistic pathogen, colonized in the human gastrointestinal tract and genitourinary tract and can cause superficial infections of mucous membranes and skin. Infection or not depends on an imbalance between enhanced Candida albicans virulence and an impaired host defense system. Recent studies have found that photodynamic therapy (PDT) can be used for antimicrobial infections. This study evaluated the ability of methylene blue (0.05 m M) -mediated sublethal PDT to alter the pathogenicity of Candida albicans and examined whether the progeny cells maintain this characteristic change. Materials and Methods Candida albicans ATCC90028 was selected as the experimental object, 50μM methylene blue as photosensitizer, the experimental light source as Ga Al As diode