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本文旨在研究丁酸钠对内毒素血症小鼠体内炎症反应、肺组织损伤及生存率的影响。Balb/c小鼠经腹腔注射丁酸钠(200 mg/kg)后,予以致死剂量脂多糖(20 mg/kg, i.p.)以诱导严重内毒素血症。造模后观察小鼠存活时间或在18 h后处死小鼠,收集血浆检测细胞因子TNF-α和IL-6水平;取肺组织检测肺组织湿/干重比、髓过氧化物酶活性,并经石蜡切片HE染色观察组织病理学改变。结果显示,丁酸钠明显减轻内毒素血症小鼠肺组织病理学损伤,降低肺组织湿/干重比及髓过氧化物酶活性。丁酸钠还可显著降低内毒素血症小鼠血浆TNF-α和IL-6水平,并提高实验动物生存率。以上结果提示,丁酸钠可有效抑制内毒素血症小鼠肺内炎症反应,提高内毒素血症小鼠生存率。
The purpose of this paper is to investigate the effect of sodium butyrate on the inflammatory response, lung injury and survival in endotoxemic mice. Balb / c mice were given a lethal dose of lipopolysaccharide (20 mg / kg, i.p.) after intraperitoneal injection of sodium butyrate (200 mg / kg) to induce severe endotoxemia. After modeling, the survival time of mice was observed or the mice were sacrificed after 18 hours. Plasma samples were collected to detect the levels of cytokines TNF-α and IL-6. Lung tissue samples were taken for lung wet / dry weight ratio, myeloperoxidase activity, Histopathological changes were observed by paraffin section HE staining. The results showed that sodium butyrate significantly attenuated pulmonary histopathology in mice with endotoxemia and decreased the wet / dry weight ratio of lung tissue and myeloperoxidase activity. Sodium butyrate can also significantly reduce the plasma levels of TNF-α and IL-6 in mice with endotoxemia and improve the survival rate of experimental animals. The above results suggest that sodium butyrate can effectively inhibit lung inflammation in mice with endotoxemia and improve the survival rate of mice with endotoxemia.