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本研究从细胞周期调控这一角度来探讨胆固醇缺乏对Ｔ淋巴细胞增殖影响的分子机制。结果显示：经去脂血清培养基培养的Ｊｕｒｋａｔ细胞加ｌｏｖａｓｔａｔｉｎ 处理３ｄ 后，３Ｈ－ＴｄＲ掺入率明显下降，免疫细胞化学染色及免疫印迹法分析表明处理后的细胞ｃｙｃｌｉｎ Ｄ１、ＣＤＫ４及ｐＲｂ 蛋白的表达明显降低，加入低密度脂蛋白（ＬＤＬ）可以部分逆转上述变化。结果提示，无论是内源性还是外源性胆固醇的缺乏都能抑制Ｊｕｒｋａｔ细胞的增殖，推测这种变化可能与ｃｙｃｌｉｎ Ｄ１、ＣＤＫ４及ｐＲｂ 蛋白表达量的下降而导致相应的ｃｙｃｌｉｎ Ｄ１／ＣＤＫ４复合活性下降有关
This study explored the molecular mechanism of the effects of cholesterol deficiency on T lymphocyte proliferation from the perspective of cell cycle regulation. The results showed that 3H-TdR incorporation decreased significantly in Jurkat cells treated with devastated serum for 3 days, and the incorporation rate of 3H-TdR decreased significantly. Immunocytochemistry and Western blot analysis showed that the cyclin D1, CDK4 and pRb proteins The expression was significantly reduced, adding low-density lipoprotein (LDL) can partially reverse the above changes. The results suggest that either endogenous or exogenous cholesterol deficiency can inhibit the proliferation of Jurkat cells, suggesting that this change may be associated with decreased cyclin D1, CDK4 and pRb protein expression resulting in the corresponding cyclin D1 / CDK4 complex activity Decline related